The method of preventing liquid phase failure
To keep pump good operating performance, must maintain system clean, ensure used solvents and reagent quality, before mobile phase entering into flow pipe, it is required to filter and degassing.
Accurate prediction prevention pump failure and timely solve appears very important.
1） using high quality reagents and HPLC level solvent is good to chromatographic column;
2） before mobile phase and solvent, using high quality materials filtering,
3） degassing to mobile phase and solvent;
4） when using every time, must exhaust vent, after work, clean the chromatographic column and pump buffer solution and pests, Don't let water and corrosive solvent and other harmful substances persisted pumps and in the chromatographic column,
5） change filtering ring and other gasket regularly online,
6） strict compliance to pump operation manual suggestions.
In good operating condition pump, should make chromatograms baseline smoothly; The retention time repeatability is good. When equal degrees elution, pressure fluctuations less than 2%. Gradient elution, pressure change should be slow and steady.
In order to remove the fault as soon as possible, peacetime should be regular prepared sealing, one-way valve (inlet and outlet), pump head device, various joints, fuse unit etc, and changing tools.
